This page will help you compare kit specs and protocols across manufacturers. Click on the product or protocol links to be directed to the manufacturers' pages.
Sigma-Aldrich | Zymo Research | |
Name | GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit | ZymoPURE™ Plasmid Maxiprep Kit |
Product No. | NA0410-1KT | D4203 Request a Sample Kit |
Price | $572.00 ($22.88 per prep) | $398.00 ($19.90 per prep) |
Size | 25 preps | 20 preps |
URL | Product link | Product link |
Specifications | ||
Format | Spin Column | Spin Column |
Main sample type | Plasmid DNA | Plasmid DNA |
Sample amount | 150 mL E. coli Culture | 150 mL E. coli Culture |
Elution Volume | 3 mL | >200 uL |
Yield | Up to 1.2 mg | Up to 1.2 mg |
Time per run or per prep | 35 minutes | 18 minutes |
# of Protocol Steps | 13 Steps | 8 Steps |
Link to Protocol | Protocol link | Protocol link |
Components | ||
RNase A Solution: 2.5 mL | ZymoPURE™ P1 (contains RNase A): 2 x 150mL | |
Resuspension Solution: 375 mL | ZymoPURE™ P2 (lysis buffer): 2 x 150mL | |
Lysis Buffer: 375 mL | ZymoPURE™ P3 (neutralization buffer): 2 x 150mL | |
Neutralization Solution: 375 mL | ZymoPURE Binding Buffer: 2 x 150mL | |
Binding Solution: 280 mL | ZymoPURE™Wash 1: 2 x 55mL | |
Wash Solution 1: 375 mL | ZymoPURE™Wash 2 (Concentrate): 2 x 23mL | |
Wash Solution 2: 75 mL | ZymoPURE™ Elution Buffer: 12 mL | |
Column Preparation Solution: 2 x 225 mL | Zymo-Spin V-P Column Assemblies: 20 | |
Endotoxin Free Water: 100 mL | ZymoPURE™ Syringe Filters: 20 | |
GenElute HP Endotoxin-Free Maxiprep Filter: 25 | ZymoPURE™ Syringe Plungers: 20 | |
VacCap: 25 | Collection Tubes: 20 | |
GenElute HP Maxiprep Binding Column: 25 | ||
Collection Tubes (50 mL): 50 |
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