This page will help you compare kit specs and protocols across manufacturers. Click on the product or protocol links to be directed to the manufacturers' pages.
| Sigma-Aldrich | Zymo Research | |
| Name | GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kit | ZymoPURE™ Plasmid Maxiprep Kit |
| Product No. | NA0410-1KT | D4203 Request a Sample Kit |
| Price | $572.00 ($22.88 per prep) | $398.00 ($19.90 per prep) |
| Size | 25 preps | 20 preps |
| URL | Product link | Product link |
| Specifications | ||
| Format | Spin Column | Spin Column |
| Main sample type | Plasmid DNA | Plasmid DNA |
| Sample amount | 150 mL E. coli Culture | 150 mL E. coli Culture |
| Elution Volume | 3 mL | >200 uL |
| Yield | Up to 1.2 mg | Up to 1.2 mg |
| Time per run or per prep | 35 minutes | 18 minutes |
| # of Protocol Steps | 13 Steps | 8 Steps |
| Link to Protocol | Protocol link | Protocol link |
| Components | ||
| RNase A Solution: 2.5 mL | ZymoPURE™ P1 (contains RNase A): 2 x 150mL | |
| Resuspension Solution: 375 mL | ZymoPURE™ P2 (lysis buffer): 2 x 150mL | |
| Lysis Buffer: 375 mL | ZymoPURE™ P3 (neutralization buffer): 2 x 150mL | |
| Neutralization Solution: 375 mL | ZymoPURE Binding Buffer: 2 x 150mL | |
| Binding Solution: 280 mL | ZymoPURE™Wash 1: 2 x 55mL | |
| Wash Solution 1: 375 mL | ZymoPURE™Wash 2 (Concentrate): 2 x 23mL | |
| Wash Solution 2: 75 mL | ZymoPURE™ Elution Buffer: 12 mL | |
| Column Preparation Solution: 2 x 225 mL | Zymo-Spin V-P Column Assemblies: 20 | |
| Endotoxin Free Water: 100 mL | ZymoPURE™ Syringe Filters: 20 | |
| GenElute HP Endotoxin-Free Maxiprep Filter: 25 | ZymoPURE™ Syringe Plungers: 20 | |
| VacCap: 25 | Collection Tubes: 20 | |
| GenElute HP Maxiprep Binding Column: 25 | ||
| Collection Tubes (50 mL): 50 | ||
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