This page will help you compare kit specs and protocols across manufacturers. Click on the product or protocol links to be directed to the manufacturers' pages.
Qiagen | Zymo Research | |
Name | EndoFree Plasmid Kits | ZymoPURE™ Plasmid Gigaprep Kit |
Product No. | 12391 | D4204 |
Price | $709 ($141.8 per prep) | $443 ($88.6 per prep) |
Size | 5 preps | 5 preps |
URL | Product link | Product link |
Specifications | ||
Endotoxin Free | Yes | Yes |
Format | Manual vacuum | Manual vacuum |
Main sample type | plasmid | plasmid |
Sample amount | 2.5L | 1L of high copy or 2.5L of low copy (Up to 25kb) |
Yield | Up to 10 mg per preparation | Up to 10 mg per preparation |
Time per run or per prep | 310 minutes | 50 min |
# of Protocol Steps | 16 steps | 12 steps |
Link to Protocol | Protocol link | Protocol link |
Components | ||
QIAGEN-tip 10000: 5 | VI-P Columns: 5 | |
QIAfilter Mega-Giga Cartridges: 5 | Giga Filter: 5 | |
Buffer P1: 3x 250ml | ZymoPURE™ P1 (Red): 2x 410ml | |
Buffer P2: 3x 250ml | ZymoPURE™P2(Green): 2x 410ml | |
Buffer P3: 3x 250ml | ZymoPURE™P3(Yellow): 2x 410ml | |
Buffer FWB2: 2x 140ml | Binding Buffer: 2x 410ml | |
Buffer QBT: 2x 200ml | Wash 1: 410ml | |
Buffer QC: 7x 500ml | Wash 2 (concentrate): 5x23ml | |
Buffer QN: 510ml | Elution Buffer: 30ml | |
Buffer ER: 200ml | 600ml Reservoir: 5 | |
Buffer TE: 110ml | ||
LyseBlue: 3x 250ul | ||
Endotoxin-free water: 17ml | ||
RNase A: 3x 25mg |
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