Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) Media Formulation Comparison

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Vendor Caisson Thermo Corning Sigma
Catalog Number DFL15 11320 10-090 8062
Ingredients (mg/L except where noted otherwise)
Glycine 18.75 18.75 18.75 18.75
L-Alanine 4.45 4.45 4.45 4.455
L-Arginine Hydrochloride 147.5 147.5 147.5 147.5
L-Asparagine-H2O 7.5 7.5 7.5 7.5
L-Aspartic Acid 6.65 6.65 6.65 6.65
L-Cysteine Hydrochloride-H2O 17.56 17.56 17.56 17.56
L-Cystine 2HCl 31.29 31.29 31.29 31.29
L-Glutamic Acid 7.35 7.35 7.35 7.35
L-Glutamine 365 365 365 365
L-Histidine Hydrochloride-H2O 31.48 31.48 31.48 31.48
L-Isoleucine 54.47 54.47 54.37 54.57
L-Leucine 59.05 59.05 58.95 59.05
L-Lysine Hydrochloride 91.25 91.25 91.35 91.25
L-Methionine 17.24 17.24 17.24 17.24
L-Phenylalanine 35.48 35.48 35.48 35.48
L-Proline 17.25 17.25 17.25 17.25
L-Serine 26.25 26.25 26.25 26.25
L-Threonine 53.45 53.45 53.55 53.45
L-Tryptophan 9.02 9.02 9.02 9.02
L-Tyrosine Disodium Salt Dihydrate 55.79 55.79 55.815 55.79
L-Valine 52.85 52.85 52.85 52.85
Biotin 0.0035 0.0035 0.00365 0.0035
Choline chloride 8.98 8.98 8.98 8.98
D-Calcium Pantothenate 2.24 2.24 2.24 2.24
Folic Acid 2.65 2.65 2.65 2.65
Niacinamide 2.02 2.02 2.0185 2.02
Pyridoxine Hydrochloride 2.031 2.013 2.031 2
Riboflavin 0.22 0.219 0.219 0.219
Thiamine Hydrochloride 2.17 2.17 2.17 2.17
Vitamin B12 0.68 0.68 0.68 0.68
i-Inositol 12.6 12.6 12.61 12.6
Calcium Chloride (CaCl2) (anhyd.) 1.05 mM 1.05 mM 1.05 mM 1.05 mM
Cupric Sulfate 0.005 uM 0.005 uM 0.005 uM 0.005 uM
Ferric Nitrate (Fe(NO3)3"9H2O) 0.05 0.05 0.05 0.05
Ferric Sulfate (FeSO4-7H2O) 0.417 0.417 0.417 0.417
Magnesium Chloride 0.3 mM 0.3 mM   0.3 mM
Magnesium Sulfate (MgSO4) (anhyd.) 0.41 mM 0.41 mM 0.71 mM 0.41 mM
Potassium Chloride (KCl) 311.8 311.8 311.8 311.8
Sodium Bicarbonate (NaHCO3) 2438 2438 2438 1200
Sodium Chloride (NaCl) 6995.5 6995.5 7000 6996
Sodium Phosphate Dibasic (Na2HPO4) anhydrous 70.96 71.02 71 71.02
Sodium Phosphate Monobasic (NaH2PO4-H2O) 0.45 mM 0.45 mM 0.45 mM 0.45 mM
Zinc Sulfate (ZnSO4-7H2O) 0.432 0.432 0.4315 0.432
D-Glucose (Dextrose) 3151 3151 3151 3150
Hypoxanthine Na 2.39 2.39 2.385 2.44
Linoleic Acid 0.042 0.042 0.042 0.042
Lipoic Acid 0.105 0.105 0.104 0.105
Phenol Red 8.1 8.1 8.1 8.63
Putrescine 2HCl 0.081 0.081 0.08 0.081
Sodium Pyruvate 55 55 110 55
Thymidine 0.365 0.365 0.365 0.365

 

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