Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) Media Formulation Comparison

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Vendor	Caisson	Thermo	Corning	Sigma
Catalog Number	DFL15	11320	10-090	8062
Ingredients (mg/L except where noted otherwise)
Glycine	18.75	18.75	18.75	18.75
L-Alanine	4.45	4.45	4.45	4.455
L-Arginine Hydrochloride	147.5	147.5	147.5	147.5
L-Asparagine-H2O	7.5	7.5	7.5	7.5
L-Aspartic Acid	6.65	6.65	6.65	6.65
L-Cysteine Hydrochloride-H2O	17.56	17.56	17.56	17.56
L-Cystine 2HCl	31.29	31.29	31.29	31.29
L-Glutamic Acid	7.35	7.35	7.35	7.35
L-Glutamine	365	365	365	365
L-Histidine Hydrochloride-H2O	31.48	31.48	31.48	31.48
L-Isoleucine	54.47	54.47	54.37	54.57
L-Leucine	59.05	59.05	58.95	59.05
L-Lysine Hydrochloride	91.25	91.25	91.35	91.25
L-Methionine	17.24	17.24	17.24	17.24
L-Phenylalanine	35.48	35.48	35.48	35.48
L-Proline	17.25	17.25	17.25	17.25
L-Serine	26.25	26.25	26.25	26.25
L-Threonine	53.45	53.45	53.55	53.45
L-Tryptophan	9.02	9.02	9.02	9.02
L-Tyrosine Disodium Salt Dihydrate	55.79	55.79	55.815	55.79
L-Valine	52.85	52.85	52.85	52.85
Biotin	0.0035	0.0035	0.00365	0.0035
Choline chloride	8.98	8.98	8.98	8.98
D-Calcium Pantothenate	2.24	2.24	2.24	2.24
Folic Acid	2.65	2.65	2.65	2.65
Niacinamide	2.02	2.02	2.0185	2.02
Pyridoxine Hydrochloride	2.031	2.013	2.031	2
Riboflavin	0.22	0.219	0.219	0.219
Thiamine Hydrochloride	2.17	2.17	2.17	2.17
Vitamin B12	0.68	0.68	0.68	0.68
i-Inositol	12.6	12.6	12.61	12.6
Calcium Chloride (CaCl2) (anhyd.)	1.05 mM	1.05 mM	1.05 mM	1.05 mM
Cupric Sulfate	0.005 uM	0.005 uM	0.005 uM	0.005 uM
Ferric Nitrate (Fe(NO3)3"9H2O)	0.05	0.05	0.05	0.05
Ferric Sulfate (FeSO4-7H2O)	0.417	0.417	0.417	0.417
Magnesium Chloride	0.3 mM	0.3 mM		0.3 mM
Magnesium Sulfate (MgSO4) (anhyd.)	0.41 mM	0.41 mM	0.71 mM	0.41 mM
Potassium Chloride (KCl)	311.8	311.8	311.8	311.8
Sodium Bicarbonate (NaHCO3)	2438	2438	2438	1200
Sodium Chloride (NaCl)	6995.5	6995.5	7000	6996
Sodium Phosphate Dibasic (Na2HPO4) anhydrous	70.96	71.02	71	71.02
Sodium Phosphate Monobasic (NaH2PO4-H2O)	0.45 mM	0.45 mM	0.45 mM	0.45 mM
Zinc Sulfate (ZnSO4-7H2O)	0.432	0.432	0.4315	0.432
D-Glucose (Dextrose)	3151	3151	3151	3150
Hypoxanthine Na	2.39	2.39	2.385	2.44
Linoleic Acid	0.042	0.042	0.042	0.042
Lipoic Acid	0.105	0.105	0.104	0.105
Phenol Red	8.1	8.1	8.1	8.63
Putrescine 2HCl	0.081	0.081	0.08	0.081
Sodium Pyruvate	55	55	110	55
Thymidine	0.365	0.365	0.365	0.365

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